Table of contents

1. Introduction 1
1.1. Insulin resistance 1
1.2. Macronutrient contributions to obesity 5
1.3. GLUT4 transporters and their regulation by membrane trafficking 7
1.4. Thyroid hormone agonists 15
1.5. Exercise-induced glucose uptake in muscle and the role of AMPK 16
1.6. Mouse model of type 2 diabetes mellitus 21
2. Statement of purpose, specific hypothesis, and specific aims 22
3. Methods 23
3.1. Reagents and cell culture 23
3.2. Mice 25
4. Results 26
4.1. Usp25m interacts with TUG 26
4.2. TUG cleavage differences in HFD and RC fed mice 27
4.3. Activated AMPK 27
5. Discussion 35
5.1. Usp25m interacts with TUG 35
5.2. HFD inhibits TUG cleavage 36
5.3. AMPK 36
References 39