Table of Contents

Abstract
Dedication
Acknowledgements
Table of Contents
List of Figures
1 Introduction
1.1 Preservatives and Disinfectants in Developmental Toxicology
1.2 Benzyl Alcohol
1.3 Zebrafish as a model for developmental toxicology studies
1.4 Zebrafish developmental stages
1.5 Oxidative Stress
1.6 Cell Death
1.7 Microtubule Formation
2 Objectives
3 Methods and Materials
3.1 Materials
3.2 Preparation of Zebrafish Embryos for Exposure to Benzyl Alcohol
3.3 Exposure of Zebrafish Embryos to Benzyl Alcohol
3.4 ZETA Assay
3.5 Acridine Orange Assay
3.6 Immunohistochemistry Assay
3.7 Superoxide Anion Assay
4 Results
5 Discussion
6 Future Investigations
References